Antigen

Antigen Class

TFs and others

Antigen

Fosb

Cell type

Cell type Class

Neural

Cell type

Nucleus Accumbens

MeSH Description

Collection of pleomorphic cells in the caudal part of the anterior horn of the LATERAL VENTRICLE, in the region of the OLFACTORY TUBERCLE, lying between the head of the CAUDATE NUCLEUS and the ANTERIOR PERFORATED SUBSTANCE. It is part of the so-called VENTRAL STRIATUM, a composite structure considered part of the BASAL GANGLIA.

Sample

source_name

nucleus accumbens

genotype

wild-type

cell type

bulk

Sex

male

treatment

saline

antibody

FosB (2251, Cell Signaling)

administration

Self-administration

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were prepared through discontinuous sucrose gradient centrifugation, isolated through INTACT approach, and subject to CUT&RUN procedures. Sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645, New England BioLabs) with NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (E7600S, New England BioLabs) based on manufacture instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

36255276

Reads aligned (%)

41.5

Duplicates removed (%)

30.0

Number of peaks

770 (qval < 1E-05)

mm9

Number of total reads

36255276

Reads aligned (%)

41.5

Duplicates removed (%)

29.9

Number of peaks

737 (qval < 1E-05)